Research Technician II

• As a community, the University of Rochester is defined by a deep commitment to Meliora - Ever Better.
• Embedded in that ideal are the values we share: equity, leadership, integrity, openness, respect, and accountability.
• Together, we will set the highest standards for how we treat each other to ensure our community is welcoming to all and is a place where all can thrive.
• Job Location (Full Address): 601 Elmwood Ave, Rochester, New York, United States of America, 14642 Opening: Worker Subtype: Regular Time Type: Full time Scheduled Weekly Hours: 40 Department: 400098 General Neurology Unit Work Shift: UR - Day (United States of America) Range: UR URG 105 H Compensation Range: $19.96 - $27.94 The referenced pay range represents the minimum and maximum compensation for this job.
• Individual annual salaries/hourly rates will be set within the job's compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.

Responsibilities

This individual will process human whole blood samples on a daily basis using the protocols already set up in the lab and keep the paperwork associated with it up to date. Initially, the candidate will conduct assigned experiments, working with daily supervision. The individual is expected to develop more independence over time in performing the experiments. It is very necessary to strictly adhere to the protocols to avoid manual errors in sample processing. ESSENTIAL FUNCTIONS Collect blood samples, isolate Peripheral Blood Mononuclear Cells (PBMCs) via density gradient centrifugation using Ficoll reagent, cryopreserve PBMCs. Maintain lab safety and cleanliness. Performs routine maintenance on equipment. Fix blood samples using 4% Paraformaldehyde and process for cell staining using intracellular as well as cell surface markers of inflammation. Acquire the stained samples using flow cytometry. Maintain the sample processing and storage log and analyze the flow cytometry data. Run enzyme-linked immunosorbent assays (ELISA) for various plasma proteins Transfer samples to the central storage facility and attend monthly project related meetings. Perform general laboratory duties such as preparing reagents and restocking supplies. Other duties as assigned. MINIMUM EDUCATION & EXPERIENCE High School diploma or GED required Associate or Bachelor’s degree in Health Science, Biology, Chemistry or other science related field of study preferred or equivalent combination of education and experience 1 year experience performing basic sampling, monitoring or research activities, compiling and collecting data and preparing reports required Previous experience with basic cell culture techniques, handling biological specimen preferred KNOWLEDGE, SKILLS AND ABILITIES Knowledge of OSHA safety requirements and scientific methods required Prior experience in flow cytometry, ELISA, whole blood processing preferred The University of Rochester is committed to fostering, cultivating, and preserving an inclusive and welcoming culture to advance the University’s Mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion, creed, sex, sexual orientation, citizenship status, or any other characteristic protected by federal, state, or local law (Protected Characteristics). This commitment extends to non-discrimination in the administration of our policies, admissions, employment, access, and recruitment of candidates, for all persons consistent with our values and based on applicable law.