Cell Assay Associate Scientist - Blue Sky Kinases

Responsibilities

Research kinase-mediated signaling pathways, kinase targets or kinase-dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects Perform appropriate cellular assays and cells in collaboration with senior cell biology expert in the team and characterize cellular phenotypes related to structure-guided perturbations in kinase regulation or function Evaluate protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT-PCR), protein ubiquitination, and protein abundance using variety of cell based assays. Develop and perform cell-based phenotypic assays, including but not limited to viability, apoptosis, autophagy, migration, or differentiation Perform optimization and troubleshooting of cell-based assays Perform statistical analysis using graphing software or R scripts Interpret, analyze, and communicate cell-based assay results within the Cell-Based Assays team and with collaborators on specific kinase projects Preferred Experience and Expertise: Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways Expertise in culturing a variety of types of cells, such as cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells Expertise in multiple methods for exogenous protein expression Proficiency in the presentation of experimental design and results using PowerPoint or similar software Experience with mouse models, preferred but not essential Preferred Technical Capabilities: Cloning, PCR, and qRT-PCR Sterile technique Cell transfection, transduction, or infection to exogenously express proteins of interest transiently or for cell line generation Quantitative immunoassays, such as ELISA, AlphaLISA, and Mesoscale Discovery Immunoprecipitation and Western blotting Protein-protein interaction analysis, such as proximity ligation, FRET, or NanoBit assays Flow cytometry and fluorescence-activated cell sorting (FACS) Reporter gene assays Phenotypic assays, such as viability, apoptosis, proliferation, migration, colony-forming, autophagy, endocytosis, secretion, or differentiation assays Microscopy, such as immunofluorescence, live-cell imaging, subcellular localization, colocalization analysis Cellular fractionation Preferred Competencies: Creative and results-driven with strong critical thinking and the ability to learn quickly Hardworking, well-organized, flexible, and capable of multitasking efficiently in a fast-paced environment Collaborative and efficient member of teams with diverse backgrounds and opinions Ability to prioritize work and complete assignments promptly Better at Work. Better at Life. When you work at St. Jude, you’ll join a highly collaborative work culture that inspires you every day to be your best. With opportunities for learning and growth, you can shape a career path that is right for you while also enjoying all the benefits and stability of working for a world-class institution. This includes work-life balance with generous paid time-off and on-campus conveniences that make life a little easier. Join us and you’ll quickly see why St. Jude is consistently ranked by our employees on Glassdoor as a “Best Place to Work.” Minimum Education and/or Training: Bachelor’s degree in a relevant scientific area Minimum Experience: 7 + years of research experience is required with a Bachelor's degree 5 + years of research experience is required with a Master’s degree 2 + years of research experience is required with a PhD Have More in Memphis Get the best of big-city amenities mixed with all the charm of a small-town feel when you live in Memphis. From our world-renowned music scene to our eclectic mix of food and people. Memphis is a place our employees are proud to call home. Our region enjoys a cost of living more than 20% lower than the national average and our state places no personal income tax on wages. Plus, Memphians spend five hours less per year in their daily commute compared to the national average. Learn how you can “Have More in Memphis.” Benefits Your health and well-being are important to us. That's why St. Jude Children's Research Hospital provides employees with high-quality benefits choices. We are committed to ensuring our benefits are competitive and affordable. St. Jude continues to cover medical premiums for employees and offers low additional costs for dependents. View all the benefits offered including Medical & Prescription Drug Plans, Dental Plan, Vision Plan, Financial Security, Retirement Plan, Living Well - Employee Wellness Program, Time Off Programs, Educational Benefits, Family Friendly Benefits, and Benefits Unique to St. Jude. Compensation In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $65,520 - $114,400 per year for the role of Cell Assay Associate Scientist - Blue Sky Kinases. Explore our exceptional benefits! St. Jude is an Equal Opportunity Employer No Search Firms St. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.