Alnylam Pharmaceuticals·Cambridge, Massachusetts
Support investigative siRNA research by applying biochemistry and molecular biology assays to evaluate RNAi compounds. Develop and validate cell-based assays to assess safety profiles and analyze complex datasets to influence drug design.
Overview The Investigative Sciences team within the Early Development Department at Alnylam is seeking a highly motivated and experienced Senior Associate Scientist to join an interdisciplinary team dedicated to expanding the reach of RNAi therapeutics through in vitro and in vivo mechanistic studies.
The role requires working knowledge in molecular and/or cellular biology, strong interpersonal skills, the capacity to multi-task, and the ability to learn and master new techniques in the field of RNAi.
We particularly seek someone who is detail-oriented and who will flourish in a goal-driven team environment under tight deadlines.
This position is onsite and will be in Cambridge, MA.
Key Responsibilities Support a variety of investigative, mechanistic siRNA research efforts to evaluate RNAi compounds by applying various biochemistry and cell/molecular biology assays.
Maintain and manipulate diverse mammalian cell lines and primary cells.
Perform transfection (lipid-based and electroporation) and free-uptake for siRNA delivery.
Quantify siRNA/mRNA/protein using various assays (qPCR, Western blotting, ELISA, immunoprecipitations, imaging).
Develop, validate, and troubleshoot cell-based assays (e.g., cell viability, reporter gene assays, and high-content imaging) to assess siRNA / monomer safety profiles.
A willingness to learn new techniques to support in vitro and in vivo studies.
Rigorously analyze complex datasets using software like GraphPad Prism or FlowJo etc.
Present experimental results clearly to cross-functional project teams to influence drug design.
BS/MS in Biology, Cell/Molecular Biology, Biochemistry or related field with 3-5 years of work experience preferably in an industrial setting, preferably in a field of oligonucleotides. Experience in mammalian cell culture and molecular biology techniques for RNA/DNA/protein quantification, ELISA, RT-qPCR and cell-based assays. Experience in Fluorescence microscopy (e.g., confocal, or live-cell imaging). Excellent lab technique and ability to work independently. Strong interpersonal and communication skills (written and oral) are required. Excellent organization, documentation, and record-keeping in ELN. Passionate about scientific discovery and eager to learn new skills and contribute to the success of the organization. #LI-JR1 U.S. Pay Range $80,000.00 - $120,000.00 The pay range reflects the full-time base salary range we expect to pay for this role at the time of posting. Base pay will be determined based on a number of factors including, but not limited to, relevant experience, skills, and education. This role is eligible for an annual short-term incentive award (e.g., bonus or sales incentive) and an annual long-term incentive award (e.g., equity). Alnylam’s robust Total Rewards package is designed to support your overall health and well-being. We offer comprehensive benefits including medical, dental, and vision coverage, life and disability insurance, a lifestyle reimbursement program, flexible spending and health savings accounts and a 401(k)with a generous company match. Eligible employees enjoy paid time off, wellness days, holidays, and two company-wide recharge breaks. We also offer generous family resources and leave. Our commitment to your well-being reflects our belief that caring for our people fuels the impact we create together. Learn more about these and additional benefits offered by Alnylam by visiting the Benefits section of the Careers website: https://www.alnylam.com/careers About Alnylam We are the leader in RNAi therapeutics – a revolutionary approach with the potential to transform the lives of people with rare and common diseases. Built on Nobel Prize-winning science, Alnylam has delivered the breakthroughs that made RNAi therapeutics possible and are just at the beginning of what’s possible. Our deep pipeline, late-stage programs, and bold vision reflect our core values: fierce innovation, passion for excellence, purposeful urgency, open culture and commitment to people. We're proud to be a globally recognized top employer, where an authentic, inclusive culture and breakthrough thinking fuel one another. At Alnylam, we commit to an inclusive recruitment process and equal employment opportunity. Qualified applicants will receive consideration for employment without regard to their sex, gender or gender identity, sexual orientation, race, color, ethnicity, national origin, ancestry, citizenship, religion, creed, physical or mental disability, pregnancy status or related conditions, genetic information, veteran or military status, marital or familial status, political affiliation, age, or any other factor protected by federal, state, or local law. Alnylam is an E-Verify Employer.
Requires a BS/MS in Biology or a related field with 3-5 years of industrial experience, preferably in oligonucleotides. Must be proficient in mammalian cell culture, RNA/DNA/protein quantification, and fluorescence microscopy.
Market context
In Massachusetts, senior associate scientist roles in biotech and drug development are typically in steady demand, especially for candidates with hands-on experience in cell-based assays and molecular biology. This role is competitive because employers often look for a BS/MS in Biology or a related field, 3–5 years of industrial experience, and practical skills in mammalian cell culture, RNA/DNA/protein quantification, fluorescence microscopy, and oligonucleotides. Review the AI-summarized requirements and benefits here to save research time, then tailor your application to show direct lab experience with those techniques.
