Research Technician

Qualifications

B.A/B.S in biological sciences, biochemistry, or a related field required. Prior laboratory research experience (including undergraduate coursework or summer research) is preferred but not required. The ideal addition to the Lindsley Laboratory will: Be kind and collegial Enjoy working in a team environment with a wide variety of faculty and staff Have good oral and written communication skills Be eager to learn new techniques and take on increasing responsibility Be able to identify potential problems and trouble-shoot solutions Possess outstanding planning, organizational, and multi-tasking skills Perform CRISPR HDR isogenic cell line generation and validation. Conduct DNA and RNA sequencing and library preparation. Analyze experimental data and interpret results to write reports and summaries of findings, including assistance with grant preparation. Maintain detailed notebook of all work-related activities. Monitor laboratory work to ensure compliance with Environmental Health and Safety information as well as the standard operation procedures specific to each laboratory. Collaborate with laboratory manager and staff scientists for the development, modification and improvement of standard operating procedures. Assist with the daily upkeep of the lab, overseeing the completion of routine lab tasks, deliveries of supplies, and maintenance of lab stocks. Cell-based telomere elongation assays Various telomere length quantification methods (qPCR, Terminal Restriction Fragment analysis, TeSLA, long-read sequencing) Such projects will integrate analysis of primary human samples with studies using engineered cell line models. Training will be provided in all relevant techniques; prior experience in tissue culture, flow cytometry, molecular biology, or small animal models is an advantage but not required. We are seeking enthusiastic, self-motivated individuals who thrive in a fast-paced, challenging research environment, and can interact well with others as part of a cohesive team. PRINCIPAL DUTIES AND RESPONSIBILITIES: The Research Technician will work under supervision to support the Lindsley Laboratory in a variety of ways: Contribute to publications as a co-author and present findings at lab meetings. Receive mentorship from the PI, postdoctoral fellows, and senior staff, with opportunities for professional development and independent project design. Process patient blood and bone marrow samples, working directly at the interface of clinical care and translational research. Maintain cells in culture and perform in vitro experiments at biological safety levels 1 and 2. Perform standard molecular biology and biochemical techniques, such as western blots, DNA/RNA isolations, PCR/qPCR, and flow cytometry. Perform telomere-specific assays, including: At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are committed to having faculty and staff who offer multifaceted experiences. Cancer knows no boundaries and when it comes to hiring the most dedicated and compassionate professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply. Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law. EEO Poster . Pay Transparency Statement The hiring range is based on market pay structures, with individual salaries determined by factors such as business needs, market conditions, internal equity, and based on the candidate’s relevant experience, skills and qualifications. For union positions, the pay range is determined by the Collective Bargaining Agreement (CBA). $47,500.00 - $51,300.00

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