Job detail

Laboratory Research Technician IV

University of Pittsburgh

#Flow Cytometry #Cloning #Biotechnology #Biology #Immunofluorescence #ELISA #FlowJo #Western Blot #Laboratory Research

Quick overview

The technician will design and perform complex laboratory procedures including T cell expansion, cell-based assays, and immunofluorescence imaging. They are also responsible for data analysis using specialized software and handling human blood samples.

Requirements summary

Candidates must possess a master's degree in Biology, Biotechnology, or a related field with at least one year of relevant experience. Proficiency in molecular biology techniques and the ability to work weekends and holidays are required.

postgraduate degreeCloningCell CultureBiochemical AssaysWestern BlotELISAFlow CytometryCytotoxicity AssaysLuminexSDS-PAGEFlowJoConfocal MicroscopyT Cell IsolationBacterial TransformationPBMC HandlingGraphpad PrismImage-J

Job description

Laboratory research technician iv The University of Pittsburgh School of Medicine seeks a Laboratory Research Technician IV to work in Pittsburgh, PA.
Duties include designing/performing: (i) isolation, activation, and expansion of T cells (ii) cryopreservation, sub-culturing, and revival of various cell lines and primary cells; (iii) cell-based assays including cytotoxicity, cell viability, and cell proliferation assays; (iv) immunofluorescence imaging using confocal microscopy and single-cell time-lapse imaging; (v) isolation and handling of PBMCs from human whole blood; (vi) multicolor flow cytometry, SDS-PAGE, western blot, multi-mode reader-based assays and various biochemical assays; and (vii) data analysis using Graphpad Prism, FlowJo, Excel and Image-J.
Must have a master’s degree (or foreign equivalent degree) in Biology, Biotechnology, or a related field plus one (1) year of experience in a related position.
Must have any experience working with: (i) Cryopreservation, sub-culturing and revival of various cell lines, mammalian cell lines and primary cells; (ii) In-vitro assays such as cytotoxicity, cell viability, cell proliferation; (iii) Live cell time-lapse imaging for cell viability assays, spheroids, immune cell killing, co-culture assays and image analysis; (iv) Isolation of and assays using Peripheral Blood Mononuclear Cells (PBMCs) from human blood; (v) isolation and expansion of naïve T cells, CD4 T cells, CD8 T cells, and regulatory T cells; (vi) Activation and expansion of T cells using Dynabeads and plate coated antigen; (vii) Screening of peptide pools, cytokine/chemokine profiling using LUMINEX, and direct, indirect, Quantikine, and sandwich ELISA assays; (viii) Seed, treat, fix, permeabilize, block and antibody staining/addition of cell lines and primary immune cells for multicolor flow cytometry of surface and intracellular markers using appropriate compensation and FMO controls for different multi-point in-vitro assays; (ix) Analysis of the flow cytometry data for receptor quantification and drug screening using Flow-Jo Software; (x) Preparing cells for molecular biology techniques such as SDS-PAGE, western Blot, protein/antibody quantification using Bradford/BCA assay and other multi-mode reader-based assays, bacterial transformation and cloning; and (xi) data analysis using Graphpad Prism and Excel.
Image analysis using Image-J.
Must be able to work weekends and holidays.
Experience can be concurrent.
Apply at https://www.join.pitt.edu, #26003389 The University of Pittsburgh is an equal opportunity employer / disability / veteran.

Laboratory Research Technician IV

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